Biosynthesis Of Copper Oxide Nanoparticles From Martynia Annua Aqueous Leaf Extract, Its Antioxidant Potential And Anti-Bacterial Ability Against Selected Wound Pathogens

Abstract

The present study report an efficient method for synthesis of copper oxide nanoparticles using aqueous leaf extract of Martynia annua. The induction of copper ion reduction process was affirmed based on the colour change of reaction mixture to deep black and the synthesis of nanoparticles was further confirmed using UV visible spectroscopy in the wavelength range of 234 nm. FTIR spectroscopy of synthesized CuO nanopoarticles reveals that the phytochemicals present in the aqueous leaf extract capped, stabilized and functionalized the nanoparticles during synthesis. The XRD analysis disseminates the crystalline nature of CuO which is propionate with increased sharpness of distinctive peaks observed during the formation of copper oxide nanoparticle. SEM image of synthesized CuO nanoparticles explain that the nanoparticles are small, well crystallinised with smooth texture of cubes and rods in shape with some degree of aggregation. The presence of moderate signal for copper ions at 3 Kev was observed during EDX analysis, which represent that copper ions are reduced during the formation of copper oxide nanoparticles. The average particle size of synthesized copper oxide nanoparticle is 112.3 nm. The PDI value of biosynthesized CuO nanoparticle is -36.2, which is associated with low particle size and least value of poly dispersity index, indicates good stability of nanoparticles. The biosynthesized nanoparticles possess well regulated DPPH scavenging activity and reducing power assay when compared to standard. The antioxidant potential of synthesized nanoparticles is entirely based on dose dependent activity. The antibacterial activity of synthesized copper oxide nanoparticle is impressive against tested wound pathogen when compared to standard. It concluded that, the synthesized nanoparticle can be incorporated as a constituent in commercial wound healing gel by following appropriate clinical trial.