GJ1158: A Salt-Inducible Host for Eco-friendly Expression of Recombinant Proteins

Abstract

The demand for recombinant protein expression is on the rise, especially for therapeutic and medical uses. However, the production of these proteins presents a significant challenge in biotechnology. The choice of expression system is critical, as it greatly influences downstream processing. E. coli is considered the most suitable bacterial system due to its rapid growth, ease of handling, low cost, and well-understood genetics. Although IPTG is commonly used for induction in E. coli, it is both environmentally harmful and expensive. In contrast, GJ1158, an E. coli strain with the T4 bacteriophage, uses an osmoresponsive operator that allows cell induction with NaCl. This makes it a cost-effective, readily available, and ecofriendly inducer. This review explores the suitability of GJ1158 as an expression host for recombinant proteins.