Possible Protective Role of Selenium Nanoparticles in Deltamethrin induced Cerebellar Toxicity in Adult Male Albino Rat: Histological, Immuno-histochemical and Biochemical Study

Abstract

Background: Deltamethrin administration induces neurotoxicity in the cerebellum through multiple mechanisms.

Aim of the Work: This study aimed to detect the adverse fluctuations occurring in the rat cerebellar cortex after deltamethrin administration. Furthermore, the possible ameliorative role of selenium nanoparticles to these changes was assessed.

Material and Methods: Forty adult male albinos’ rats were divided into four groups (Gps): Gp I (Control), Gp II (sham), Gp III (Deltamethrin), Gp IV (Deltamethrin+ Selenium). Blood samples were taken by cardiac puncture for using in biochemical analyses. The cerebellum was processed for histological, immunohistochemical &biochemical analysis.

Results: The light microscopic analysis of deltamethrin group (III) revealed that the Purkinje cells were primarily affected by the neurotoxic effects of deltamethrin among the cerebellar cortex cells. In addition to demyelination of nerve fibers. A strong positive GFAP immunoreaction was observed in this group. On the other aspect, deltamethrin with selenium nanoparticles treated group (IV) showed obvious protection in the cerebellar histological architecture and weak positive GFAP immunoreaction. Biochemically, the cerebella of deltamethrin group (III) illustrated statistically significant increase in the level of MDA and a significant decrease in the level of GSH and the activity of SOD compared to statistically significant decrease in Gp IV (Deltamethrin+ Selenium).

Conclusion: In light of those observations, the current study revealed that selenium nanoparticles have protective role from the deleterious effects of deltamethrin on the cerebellar architecture, oxidative status and apoptosis.