Investigation of the Gene Expression of APOAI and Gene Variants in ABCAI Gene in Type 2 Diabetic Pakistani Patients with and without Dyslipidemia.
DOI:
https://doi.org/10.64149/J.Ver.8.18s.469-475Keywords:
Apolipoprotein I gene, ATP- binding cassette transporter I (ABCAI) gene, Dyslipidemia, Type 2 Diabetes Mellitus, High Density Lipoprotein (HDL-C)Abstract
Objective: To determine the APOAI gene expression and genetic variations in ABCAI gene in type two diabetes mellitus (T2DM) patients with and without dyslipidemia and association with HDL-C profile.
Methodology: A cross sectional analytical study was conducted on ninety subjects; comprised of diabetic dyslipidemics, diabetics without dyslipidemia and healthy subjects, who were randomly selected. RNA and genomic DNA were isolated from human peripheral blood samples. The APOAI gene and ABCAI gene were amplified through polymerase chain reactions. APOAI gene RNA expression analysis on real time PCR and relative quantification via ∆∆CT method were carried out. DNA sequencing of ABCAI gene was performed and analyzed using BioEdit 7.9 biological software for detection of genetic mutations.
Results: The mRNA abundance of APOAI gene was found changed in diseased groups compared to control. The expression of APOAI was 5.3fold changed in diabetic dyslipidemia group and 6.9fold changed in diabetic group compared to control group. There was no genetic variation found in Exon-4 of the ABCAI gene and DNA sequence was in normal pattern.
Conclusion: The normalized expression of APOAI gene in diabetic dyslipidemia and diabetic group was up-regulated compared to the control group and the underlying cause might be diabetes.
