In Vivo Evaluation Of Phytosomes Containing Rhaphidophora Aurea And Plectranthus Barbatus For The Treatment Of Anxiety

Abstract

This study investigates the in vivo anxiolytic efficacy of phytosomal formulations containing Rhaphidophora aurea and Plectranthus barbatus extracts in Swiss albino mice (Mus musculus). Thirty-six male mice (6–8 weeks, 25–30 g) were divided into six groups (n=6): control (saline), disease (negative control), Diazepam (standard), and low-, medium-, and high-dose phytosomal formulations (F3–F5). Anxiolytic effects were assessed using the Elevated Plus Maze (EPM), Open Field Test (OFT), and Light-Dark Box (LDB) tests, measuring parameters such as time spent in open/light zones, locomotor activity, and transitions. Results demonstrated a dose-dependent anxiolytic effect, with the high-dose phytosome (F5) exhibiting significant improvements in open-arm time (72.4 sec, EPM), central zone duration (78.1 sec, OFT), and light compartment exploration (141.6 sec, LDB), closely resembling Diazepam’s effects (78.3 sec, 85.4 sec, and 148.7 sec, respectively). The disease group showed pronounced anxiety-like behavior across all tests. Statistical analysis (one-way ANOVA, Dunnett’s post-hoc, p<0.05) confirmed the significance of these findings. Phytosomal encapsulation enhanced the bioavailability and anxiolytic potential of the herbal extracts, suggesting their therapeutic promise as a safer alternative for anxiety management.