Anxiolytic Prospective of Limonene Terpenoids-A Preclinical Investigation

Abstract

Anxiety is a common and often disabling mental health issue affecting millions globally. Traditional plant-based remedies have gained attention as safer alternatives to conventional medications. In this study, we investigated the anti-anxiety potential of biosynthesized silver nanoparticles (Ag-NPs) derived from Abies pindrow Royle leaf extracts limonene. Using a green synthesis method, Ag-NPs were prepared and characterized. Their effects were evaluated in a rodent model of anxiety using behavioral tests (Elevated Plus Maze), biochemical markers of oxidative stress, and brain histology. The results demonstrated that Abies pindrow- terpenoids derived Ag-NPs significantly reduced anxiety-like behaviors, lowered oxidative stress levels, and showed protective effects on brain tissues. These findings suggest that green-synthesized Ag-NPs from Abies pindrow Royle may represent a promising natural therapeutic approach for managing anxiety.